Zeziphi iidayi ezisetyenziswa kakhulu ekunqunqeni izinto eziphilayo?

Kukho iintlobo ngeentlobo zeedayi ezisetyenziswa kakhulu kwi-bioslicing, nganye kuzo ineempawu ezahlukeneyo kunye noluhlu lwezicelo. Nazi ezinye zeedayi ze-bioslicing ezisetyenziswa rhoqo kunye nentshayelelo yazo emfutshane:

Okokuqala, iidayi zendalo

IHematoxylin: Le yipigment ethathwe kumasebe omileyo e-hematoxylum yaseMzantsi Melika (i-legume yasetropiki) ngokuyifaka kwi-ether. IHematoxylin ayinakudayiswa ngqo, kwaye kufuneka i-oxidized ukuze ibe yi-oxyhematoxylin (ekwabizwa ngokuba yi-hematoxylin) ngaphambi kokuba isetyenziswe. Yinto elungileyo yokufaka idayi kwi-nucleus kwaye inokwahlula izakhiwo ezahlukeneyo kwiseli zibe yimibala eyahlukeneyo.

I-Carmine: I-Carmine, eyaziwa ngokuba yi-carmine okanye i-carmine, yenziwe ngeebhungane ze-cochineal ezibhinqileyo zaseTropiki ezomileyo zaza zagaywa zaba ngumgubo, zakhutshwa izinambuzane ezibomvu, zaza zanyangwa nge-alum ukuze zenziwe. I-Carmagenta ikwayidayi elungileyo kwi-nucleus, kwaye isampuli edayiweyo ayilula ukuyicima, ingakumbi xa idaywa yonke into encinci.

Okwesibini, iidayi zokwenziwa

I-Acid fuchsin: I-Acid fuchsin yidayi ene-asidi, umgubo obomvu, iyanyibilika emanzini, iyanyibilika kancinci kwi-alcohol. Yiarhente elungileyo yokudaya iiseli, isetyenziswa kakhulu ekulungiseleleni izilwanyana, ekulungiseleleni izityalo ulusu, i-pulp kunye nezinye iiseli ze-parenchyma kunye neendonga ze-cellulose.

Ubomvu baseCongo: Ubomvu baseCongo yidayi eneasidi, ekwimo yomgubo obomvu wejujube, enyibilikayo emanzini nasetywala, eluhlaza okwesibhakabhaka kwiasidi. Ihlala isetyenziswa kwimveliso yezityalo njenge-liner ye-hematoxylin okanye ezinye iidayi zeeseli, kwaye ingasetyenziselwa ukungcolisa i-cytoplasm kunye ne-nerve axes.

Uluhlaza oluqinileyo: Uluhlaza oluqinileyo yidayi eneasidi, enyibilikayo emanzini nasetywala. Luhlobo lwearhente yokudaya kwizicubu zeseli zeseli zeseli ezineplasma, esetyenziswa kakhulu kwiiseli zokudaya kunye nezicubu zezityalo.

ISudan III: ISudan III yidayi ye-asidi ebuthathaka, umgubo obomvu, enyibilikayo kumafutha kunye notywala. Libala lamafutha elihlala lisetyenziswa ukubonisa umxholo wamafutha kwizicubu.

I-Eosin: Kukho iintlobo ezininzi ze-eosin, kwaye i-eosin Y esetyenziswa kakhulu yidayi ene-asidi, ebomvu enekristale ezincinci eziluhlaza okwesibhakabhaka okanye umgubo omdaka. I-Eosin isetyenziswa kakhulu ekulungiseleleni izilwanyana, yidayi elungileyo ye-cytoplasmic, kwaye idla ngokusetyenziswa njengedayi edibanisa i-hematoxylin.

I-fuchsin esisiseko: i-fuchsin esisiseko yidayi ye-alkaline, esetyenziswa kakhulu kwimveliso yezinto eziphilayo kwaye ingasetyenziselwa ukungcolisa imicu ye-collagen kunye nemicu ye-elastic.

I-Crystal violet: I-Crystal violet yidayi ye-alkaline, esetyenziswa kakhulu kwi-cytology, histology kunye ne-bacteriology, libala elihle, elihlala lisetyenziselwa ukudaya i-nuclear.

I-Gentian violet: I-Gentian violet ngumxube weedayi ze-alkaline, ikakhulu umxube we-crystal violet kunye ne-methyl violet, engasetyenziswa ngokutshintshana ne-crystal violet xa kuyimfuneko.

Ezi dayi zidlala indima ebalulekileyo ekusikeni i-bioslicing, kwaye ngeendlela ezahlukeneyo zokufaka imibala kunye nokudibanisa, zinokubonisa ngokucacileyo ulwakhiwo lwe-morphological lweeseli kunye nezicubu, zibonelela ngenkxaso yobugcisa ebalulekileyo kuphando lwebhayoloji kunye nolwezonyango.