Njengesixhobo esichanekileyo sokujonga ihlabathi le-microscope lamacandelo ebhayoloji, iiseli, iibhaktheriya, njalo njalo, indlela yokusebenzisa kunye namanyathelo okhuseleko abaluleke kakhulu kubavelisi bamacandelo ebhayoloji. Oku kulandelayo sisishwankathelo esifutshane sokusetyenziswa kunye namanyathelo okhuseleko eemakroskopu zebhayoloji ezabelwana ngazo ngabavelisi be-microtome zebhayoloji:
Indlela yokusebenzisa
Inqanaba lokulungiselela: Beka imakroskopu kakuhle ebhentshini ukuqinisekisa ukuba ukukhanya okukufutshane kulungile. Thatha imakroskopu ngezandla zombini, ubambe ingalo ngesandla esinye kunye nesiseko ngesinye ukuze uphephe ukungcangcazela.
Ukufakela nokulungisa iimpazamo: Faka i-eyepiece kunye ne-objective, jika i-converter ukuze ilungelelanise i-objective enamandla aphantsi kunye nomngxuma wokukhanya. Lungisa i-aperture kunye nesibuko ukuze ubone umbono ocacileyo nokhanyayo.
Beka isampuli: Beka isilayi sebhayoloji kwiqonga lokulayisha kwaye usilungise nge-press clip ukuqinisekisa ukuba isampuli ijonge embindini womngxuma wokukhanya.
Lungisa ubude befocal: Sebenzisa i-coarse parafocal spiral ukuze wehlise kancinci ityhubhu ide injongo isondele kwisampuli (thintela ukudibana ngqo). Emva koko jika i-coarse quasi-focal spiral kwelinye icala, yenza i-lens barrel inyuke kancinci, kwaye ujonge utshintsho lwento ekwi-eyepiece. Xa umfanekiso ucacile ekuqaleni, ulungiswa kakuhle nge-fine parifocal spiral ukuze ufumane ukucaca.
Ukujonga nokurekhoda: Emva kokufumana into yokujonga enelensi enamandla aphantsi, ungatshintsha kancinci uye kulensi enamandla aphezulu okanye ilensi yeoyile ukuze ufumane uphononongo oluneenkcukacha ezithe vetshe. Ngexesha lenkqubo yokujonga, qwalasela ukulungisa ukukhanya komthombo wokukhanya kunye nobukhulu bomngxuma ukuze ufumane isiphumo esingcono sokujonga. Kwangaxeshanye, yenza iirekhodi zokujonga ukuze uhlalutye okulandelayo.
Imiba efuna ingqwalasela
Ukuphathwa kokukhanya: Thatha kwaye ubeke isenzo semakroskopu kufuneka sibe lula, kuphephe ukungcangcazela nokusebenza ngogonyamelo, ukuthintela umonakalo kwiindawo ezibonakalayo.
Ukusebenza okuqhelekileyo: Ngokuhambelana ngqo neenkqubo zokusebenza, okokuqala kufuneka kukhuliswe kancinci kuze kukhuliswe kakhulu, kuthintelwe ukusetyenziswa ngokuthe ngqo kwezipili zokukhulisa okuphezulu ukuze kufunyanwe ithagethi, ukuze kuncitshiswe ukuguguleka kwemakroskopu.
Ukukhuselwa kwelensi: Xa ujonga isampuli yeyeza elilulwelo, kufuneka igqunywe ngesilayidi okanye ibekwe kwisitya sepetri ukuze kuthintelwe ukugqwala okubangelwa kukudibana ngqo kolwelo nelensi. Emva kokusebenzisa isibuko seoli, hlamba amabala eoli kwilensi ngexesha elifanelekileyo.
Ukugcinwa rhoqo: Jonga kwaye ugcine imakroskopu rhoqo ukuze ihlale icocekile kwaye yomile ukuze yandise ubomi bayo bokusebenza.
Ukusetyenziswa ngokukhuselekileyo: Xa usebenzisa imakroskopu, qaphela ukhuseleko lombane, ukuze uphephe ukutshayiswa ngumbane nezinye iingozi. Kwangaxeshanye, thobela imithetho nemigaqo yelabhoratri ukuqinisekisa ukhuseleko kunye nocwangco lwenkqubo yovavanyo.
Iithegi ezinxulumene noko: I-Biopexy, abavelisi be-Biopexy, i-Biopexy, abavelisi beemodeli zesampulu,
Ixesha lokuthumela: Agasti-06-2024