Kwicandelo le-biosectioning, i-smear kunye ne-mounting ziingcamango ezimbini ezahlukeneyo, kwaye umahluko wazo uxhomekeke kakhulu kwindlela isampuli ecutshungulwa ngayo kunye nendlela icandelo elilungiselelwe ngayo.
Ukutshiza: Ukutshiza kubhekisa kwindlela yokulungiselela yokufaka isampuli ngqo kwisilayidi. Ngokwesiqhelo ii-smears zifakwa kwiisampuli zolwelo okanye kwiisampuli zeeseli, ezifana negazi, ulwelo lwe-cerebrospinal, umchamo, njl. Xa kulungiswa i-smear, isampuli iyasuswa ize ifakwe ngqo kwisilayidi, emva koko igqunywe ngesinye isilayidi ukwenza iphepha lokucinezela, elidaywa yindlela ethile yokutshiza. Ii-smears zihlala zisetyenziselwa i-cytology ukujonga imo yeseli kunye nesakhiwo kwisampuli.
Ukulayisha: Ukulayisha kubhekisa kwindlela yokulungiselela yokulungisa isampuli yethishu, ukuyisika ibe ziziqwenga ezibhityileyo nge-microtome, uze unamathisele ezi ziqwenga kwisilayidi. Ngokwesiqhelo, ukufaka kufanelekile kwiisampuli zethishu eziqinileyo, ezifana neziqwenga zethishu, iibhloko zeeseli, njl. Xa kulungiswa ukufakwa, isampuli kuqala ilungiswe, ikhutshwe amanzi emzimbeni, ifakwe kwi-wax, njl., ize inqunyulwe ibe ziziqwenga ezibhityileyo yi-microtome, ize emva koko ezi ziqwenga zincamathiselwe kwisilayidi ukuze zidaywe. Umzobo uhlala usetyenziselwa uvavanyo lwe-histological ukujonga ulwakhiwo lwethishu kunye notshintsho lwe-pathological.
Ngoko ke, isitshixo sokwahlula phakathi kwe-smear kunye nokulayisha sikwinkqubo yokuphatha kunye nokulungiselela isampuli. I-Smear yindlela yokulungiselela yokufaka isampuli ngqo kwislayidi, efanelekileyo kwiisampuli zolwelo okanye iisampuli zeseli; I-Loading yindlela yokulungiselela yokusika isampuli yezicubu eziqinileyo ibe ziziqwenga ezibhityileyo uze uyincamathisele kwislayidi, efanelekileyo kwiisampuli zezicubu eziqinileyo.
Ixesha lokuthumela: Agasti-10-2024